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Development and Validation of HPLC Methods for Analytical and Preparative Purposes

Liquid chromatography (LC) is a separation method of great importance to the chemical, pharmaceutical and biotechnological industry. The principle is that a sample of a solution of the substances is injected into a column of an porous material (stationary phase) and a liquid (mobile phase) is pumped through the column. The separation of substances is based on differences in rates of migration through the column arising from different partition of the substances between the stationary and the mobile phase. Depending on the partition behavior of the different types of substances, these will elute at different times from the column outlet.

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