Advances in Chromatography, Volume 35

The rapidly expanding growth of the literature on chromatography, capillary electrophoresis, field flow fractionation, and other separation techniques makes it difficult for any individual to maintain a coherent view of progress in the field. Rather than attempt to read the avalanche of original research papers, investigators trying to preserve even a modest awareness of advances must rely upon authoritative surveys. Featuring reliable, up-to-the-minute reviews of major developments in chromatography and other separation techniques, this critically praised series separates the most important advances from an overabundance of supplementary materials.

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Gas Chromatography in Biology and Medicine

IN the preface to the first edition of one of the earliest books on chromatography, Principles and Practice of Chromatography (Zechmeister and Cholnoky, 1943) there is written: “Every scientific advance is an advance in method.” It is also stated in this prefcice that “the invention of a new specialized laboratory procedure brings about rapid conquests in new fields of science and technology, finally it exhausts itself and is replaced by a still more practical method. The method of chromatographic adsorption invented by the talented Russian botanist, Professor M. Tswett makes possible spatial separation of components of a mixture. It is just now at the beginning of a bullish development : it offers a simple experimental procedure to the investigator especially in the fields of both pure and applied organic chemistry, of biochemistry and of physiology.”

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Illustrated Pocket Dictionary of Chromatography

Provides concise definitions, illustrations, formulas, and all of the other information that you need in a laboratory chromatography reference

The Illustrated Pocket Dictionary of Chromatography offers the perfect quick reference to the parameters, systems, and components that support successful chromatographic separations. Concise, practical, and filled with detailed drawings, diagrams, and photographs, this pocket dictionary is designed for easy use in the laboratory. It provides a complete, up-to-date reference on analytical techniques such as gas chromatography, high-performance liquid chromatography, thin layer chromatography, and capillary electrophoresis as well as preparation techniques such as solid phase extraction and microextraction.

The Illustrated Pocket Dictionary of Chromatography features:

• Explanations of key concepts
• Equations and formulas
• Parameters
• High-quality photographs of instruments, equipment, and components
• References for further research
• Explanations of common acronyms

Ideal for the working chemist or technician in pharmaceutical, industrial, forensic, government, or other lab settings, the Illustrated Pocket Dictionary of Chromatography is also appropriate as a supplemental reference for analytical chemistry courses and labs. Students and analysts alike will find this compact yet comprehensive resource a valuable companion to their work in chromatography.

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Analytical Gas Chromatography, Second Edition

Gas chromatography remains the world's most widely used analytical technique, yet the expertise of a large proportion of chromatographers lies in other fields. Many users have little real knowledge of the variablesin the chromatographic process, the interaction between those variables, how they are best controlled, how the quality of their analytical results could be improved, and how analysis times can be shortened to facilitate the generation of a greater numberof more reliable results on the same equipment. An analyst with a more comprehensive understanding of chromatographic principles and practice, however, can often improve the quality of the data generated, reduce the analytical time, and forestall the needto purchase an additional chromatograph or another mass spectrometer.The Second Edition of Analytical Gas Chromatography is extensively revised with selected areas expanded and many new explanations and figures. The section on sample injection has been updated to include newer concepts of split, splitless, hot and cold on-column, programmed temperature vaporization, and large volume injections. Coverage of stationary phases now includes discussion, applications, and rationale of the increased thermal and oxidative resistance of the newly designed silarylenepolysiloxane polymers. Conventional and"extended range"polyethylene glycol stationary phases are examined from the viewpoints of temperature range and retention index reliabilities, and the chapter on"Variables"has been completely rewritten. The ways in which carrier gas velocity influences chromatographic performance is considered in detail, and includes what may be the first rational explanation of the seemingly anomalous effects that temperature exercises on gas viscosity (and gas flow). The practical effects that these changes cause to the chromatography is examined in pressure-, flow-, and"EPC-"regulated systems."Column Selection, Installation, and Use"has been completely rewritten as well. The accuracy of theVan Deemter plots has been greatly enhanced; a new program corrects for the first time for the changes in gas density and diffusion that occur during the chromatographic process because of solute progression through the pressure drop of the column. A new section has also been added on meeting thespecial requirements of columns destined for mass spectral analysis. The chapter on"Special Applications"has been expanded to include considerations of"selectivity tuning,"of fast analysis, and the section of Applications has been thoroughly updated and expanded.

Key Features:

* Incorporates nearly 60% new material
* Covers the newest concepts and materials for sample injection and stationary phases
* Presents detailed consideration of the influence of carrier gas velocity on practical aspects of chromatographic performance
* Contains a chapter on "Special Analytical Techniques" which includes consideration of selectivity tuning and fast analysis
* Provides a new section addressing the special requirements of columns to be used in mass spectral analysis
* Includes an improved program that greatly enhances the accuracy of the Van Deemter plots by more accurately depicting localized chromatographic conditions at each point in the column.

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Advances in LC-MS Instrumentation, Volume 72

The different LC-MS techniques available today were developed to suit specific analytical needs and the application range covered by each one is wide, but still limited. GC amenable compounds can be all analyzed with a single GC-MS system whereas HPLC applications call for specific LC-MS instrumental arrangements. ESI, APCI, APPI, and EI are ionization techniques that can be combined with different analyzers, in single or tandem configuration, to create the ultimate system for a certain application. Once approaching LC-MS for a specific need, the fast technical evolution and the variegated commercial offer can induce confusion in the potential user.

The role of this book is to enlighten the state-of-the-art of LC-MS evolution through a series of contributions written by the people that brought major, recent innovations in the field. Each chapter will take into consideration the novelties, the advantages and the possible applications covered by a particular technical solution. The book will also include new analytical methods that can provide benefits using the most recent innovations in LC-MS plus a certain number of key applications.

- Contains contributions from major innovators in the field
- Covers the latest developments in the field of LC-MS
- Gives a clear outline on the advantages of various techniques and their applications

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Liquid Chromatography Column Theory

Offers comprehensive coverage of all the theoretical aspects of chromatography columns beginning with very simple concepts which describe their properties. Topics include peak capacity, column overload, thermal effects and many other chromatographic phenomena. Provides in-depth discussions of both the plate and rate theories as well as the design of the optimum column, analytical packed and open tubular columns. Includes practical examples of the use of these theories and equations derived from them.

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Modern Practice of Gas Chromatography

The bible of gas chromatography-offering everything the professional and the novice need to know about running, maintaining, and interpreting the results from GC Analytical chemists, technicians, and scientists in allied disciplines have come to regard Modern Practice of Gas Chomatography as the standard reference in gas chromatography. In addition to serving as an invaluable reference for the experienced practitioner, this bestselling work provides the beginner with a solid understanding of gas chromatographic theory and basic techniques.
This new Fourth Edition incorporates the most recent developments in the field, including entirely new chapters on gas chromatography/mass spectrometry (GC/MS); optimization of separations and computer assistance; high speed or fast gas chromatography; mobile phase requirements: gas system requirements and sample preparation techniques; qualitative and quantitative analysis by GC; updated information on detectors; validation and QA/QC of chromatographic methods; and useful hints for good gas chromatography.
As in previous editions, contributing authors have been chosen for their expertise and active participation in their respective areas. Modern Practice of Gas Chromatography, Fourth Edition presents a well-rounded and comprehensive overview of the current state of this important technology, providing a practical reference that will greatly appeal to both experienced chomatographers and novices.

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Handbook of Pharmaceutical Analysis by HPLC, Volume 6

High pressure liquid chromatography frequently called high performance liquid chromatography (HPLC or, LC) is the premier analytical technique in pharmaceutical analysis and is predominantly used in the pharmaceutical industry. Written by selected experts in their respective fields, the Handbook of Pharmaceutical Analysis by HPLC Volume 6, provides a complete yet concise reference guide for utilizing the versatility of HPLC in drug development and quality control. Highlighting novel approaches in HPLC and the latest developments in hyphenated techniques, the book captures the essence of major pharmaceutical applications (assays, stability testing, impurity testing, dissolution testing, cleaning validation, high-throughput screening,

* A complete reference guide to HPLC
* Describes best practices in HPLC and offers 'tricks of the trade' in HPLC operation and method development
* Reviews key HPLC pharmaceutical applications and highlights currents trends in HPLC ancillary techniques, sample preparations, and data handling

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Sample Preparation in Chromatography

Sample preparation is an essential step in many analyses. This book approaches the topic of sample preparation in chromatography in a methodical way, viewing it as a logical connection between sample collection and analytical chromatography. Providing a guide for choosing the appropriate sample preparation for a given analysis, this book describes various ways to process the sample, explaining the principle, discussing the advantages and disadvantages, describing the applicability to different types of samples, and showing the fitness to specific chromatographic determinations.

The first part of the book contains an overview of sample preparation showing its relation to sample collection and to the core chromatographic analysis. The second part covers procedures that do not use chemical modifications of the analyte and includes methods for sample dissolution, concentration and cleanup designed mainly for modifying the initial matrix of the sample. This part starts with conventional separations such as filtration and distillation and finishes with more advanced techniques such as solid phase extraction and electroseparations. The third part gives a description of the chemical modifications that can be performed on a sample either for fractionation purposes or to improve a specific property of the analyte. This part includes derivatizations, polymer chemical degradations, and pyrolysis.

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http://rapidshare.com/files/122767475/Sam_pre_chrom.zip

Introduction to Modern Liquid Chromatography-2 Ed.

This Second Edition incorporates the latest developments in the practical application of LC. Covers the basic of LC, the six LC methods and their applications, and various specialized areas. Provides material for in-depth comprehension of how HPLC is performed, what the necessary materials are, and possible applications. Offers thorough treatment of such areas as quantitative and qualitative analysis by HPLC, preparative scale separations, gradient elution and column-switching, sample pretreatment and reaction detectors, automated systems for high-volume testing and/or samples requiring pretreatment, troubleshooting and sample artifacts, and selection and development of LC method for a particular applications.

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http://rapidshare.com/files/87722309/Intro_mod_Liq_Chrom.zip

A Century of Separation Science

A Century of Separation Science presents an extensive overview of the critical developments in separation science since 1900, covering recent advances in chromatography, electrophoresis, field-flow fractionation, countercurrent chromatography, and supercritical fluid chromatography for high-speed and high-throughput analysis.

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http://rapidshare.com/files/75549112/Century_Separation_Science.zip

Thin Layer Chromatography : A Modern Practical Approach (Rsc Chromatography Monographs)

Thin-Layer Chromatography (TLC) is a modern, reliable tool that complements other chromatographic techniques. This book provides a practical guide to the basic principles, procedures, and pitfalls on the practical application of TLC.

Thin Layer Chromatography: A Modern Practical Approach offers a sequence of chapters following the steps of the technique as the chromatographer would follow them. The chapters provide a choice of sorbent best suited to the separation intended, followed by pre-treatment required for the sample, applying the sample to the sorbent layer, development procedure, visualisation and detection, and finally quantification. Imaging and hyphenation techniques are described. The reasons why recommendations are made for specific and more general methods are covered. The book also provides an overview of some recent developments in the field.

This practical guide is an invaluable introduction to Thin-Layer Chromatography, and will be welcomed by undergraduate students and the practising chromatographer in industry and academia.

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http://rapidshare.com/files/127763754/Tl_Chro.zip

HPLC: A Practical Guide (Chromatography Monographs)

This book answers two basic questions regarding chromatography; it focuses on how compounds can high-performance liquid be separated and on why particular compounds are separated by liquid chromatographic methods. It consists of six chapters: Basic Concepts of HPLC; Instrumentation: Preparation, Testing and Selectivity of Stationary Phase Materials; Selection of the Eluent; Separation Based on an Improved Column Efficiency; Influence of
Physical Chemistry on Separations in Liquid Chromatography. The focus is on the basic considerations in liquid chromatography rather than the applications but there are also sections on trouble-shooting. The book is concerned mainly with the selection of a packing material and the preparation of the eluent. Theoretical optimization is demonstrated using the chromatography of simple chemicals, to aid the understanding of liquid chromatography by graduate students. The examples given go beyond what can be found in general textbooks
on liquid chromatography. The separations can be easily understood from the differences in properties of familiar compounds known to undergraduate students.
The chromatographic separations described are based on solubility using molecular properties. The selection of chromatographic mode and column is analysed using solubility parameters. The separation factor cc is described in detail by physical and chemical parameters. Discussion of instrumentation focuses on high-efficiency operation and is concerned with degassing, the time constant, flow cell design, connectors and trouble-shooting. Packing materials and their synthesis and surface modification, liquid chromatographic analysis of surface activity and the evaluation of packed columns, including the measurement of void volume, are also considered.

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http://rapidshare.com/files/75526960/HPLC_Practical_Guide.zip

Handbook of Process Chromatography, Second Edition: Development, Manufacturing, Validation and Economics

This book will update the original edition published in 1997. Since the publication of the first edition, the biotechnology and biologics industries have gained extensive knowledge and experience in downstream processing using chromatography and other technologies associated with recovery and purification unit operations. This book will tie that experience together for the next generation of readers.

Updates include:

- sources and productivity
- types of products made today
- experiences in clinical and licensed products
- economics
- current status of validation
- illustrations and tables
- automated column packing
- automated systems

New topics include:

- the use of disposables
- multiproduct versus dedicated production
- design principles for chromatography media and filters
- ultrafiltration principles and optimization
- risk assessments
- characterization studies
- design space
- platform technologies
- process analytical technologies (PATs)
- biogenerics
- comparability assessments

Key Features:

- new approaches to process optimiaztion
- use of patform technologies
- applying risk assessment to process design

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http://rapidshare.com/files/74624321/Process_Chromatography_2nd_Ed.zip

Large-Scale Adsorption and Chromatography, Volumes 1-2

This book presents a unified, up-to-date development of operating methods used for large-scale adsorption and chromatography. It groups together the operating methods which have been used or studied for large-scale applications. These methods have been classified and compared. The main unifying principle has been to use the same theory, the solute movement or local equilibrium theory, to present all of the methods. Mass transfer and dispersion effects are included with the nonlinear mass transfer zone (MTZ).

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http://rapidshare.com/files/54392276/LS_AdsorptionChromatoV1-2.zip

Column Handbook for Size Exclusion Chromatography

Column Handbook for Size Exclusion Chromatography is the first comprehensive reference to provide everything one needs to know about commercial analytical and preparative columns for size exclusion and gel filtration chromatography (SEC and GFC). SEC is now widely used as a quality assurance method in the polymer industry (both synthetic and biopolymers) to determine molecular weight and molecular weight distribution. The Handbook contains contributions from every column manufacturer around the world and from many experienced column users. It covers the technology, characterization, application, evaluation, maintenance, and quality control of analytical and preparative columns for SEC and GFC. Also included are columns for two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography.

Key Features
* Evaluate and select columns with confidence for specific applications
* Optimize separations and improve the ruggedness of analytical methods
* Extend the service time of a column
* Establish a quality-control program to ensure consistency in column performance
* Avoid the expense of column damage or purchases that do not give the expected results.

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http://rapidshare.com/files/49734133/Column_Handbook_for_Size_Exclusion_Chromatography.rar

Gas Chromatography and Mass Spectrometry

This guide provides, under one cover, a wealth of practical information designed to facilitate the effectiveness of the GC/MS user. Separation conditions for numerous compound types are provided along with derivatized and underivatized compounds. A section on how to interpret mass spectral data, an extensive correlation of ion masses and neutral losses with possible structures, and examples of mass spectra are provided to further aid structure determination. Also included are basic information on instrumentation, ionization methods, quantitation, tips on the operation of mass spectrometers, the best derivatization procedures for a variety of compound types, troubleshooting techniques, and a variety of other information found to be useful to the practicing user of GC/MS instrumentation. This guide would be immediately valuable to the novice as well as the experienced GC/MS user who may not have the breadth of experience covered in this book.

Key Features:
* Condenses and organizes recent and essential information for new and experienced GC/MS users
* Comprehensively indexed and referenced
* Includes practical methods of analysis
* Serves as a text reference for short practical courses on the subject

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http://rapidshare.com/files/47056420/GC_MS.zip

Applied Thin-Layer Chromatography: Best Practice and Avoidance of Mistakes

Thin layer chromatography (TLC) is a powerful, fast and inexpensive analytical method. It has proven its usefulness in pharmaceutical, food and environmental analysis. This new edition features a completely revised chapter on documentation, now including the use of digital cameras. Selected new sorbents and instruments are also introduced.

Why has the prior edition been successful? All steps of the analytical procedure are clearly explained, starting with the choice of a suitable TLC technique and ending with data evaluation and documentation. Special emphasis is put on the proper choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering e. g. precoated layers, solvents and developing chambers, and including information on suppliers. Many practical hints for trouble shooting are given. All this is illustrated with numerous coloured figures.

How to use TLC in compliance with GLP/GMP regulations is described in detail, including the required documentation. Therefore the reader can very easily compile his own standard operating procedures.

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http://rapidshare.com/files/43933108/Applied_Thin-Layer_Chromatography.zip